any body messed around with tissue culture

[TheHerbalEssence]

 

Originally Posted by Butcher Bob

Please explain this.....cuz I would disagree.

Well, well. Maybe its not true in all instances, but I believe if you are not selectively breeding/cloning, you may tend to lose some of the positive traits of buds..

I'll run you through my experience..

Back in the day, I was working with a bunch of random bag seed. After weeding out the strains that werent so good, I was left with a pretty decent strain, but nothing special..(I actually just recently got rid of my mother plant, bud I'll come back to this in a second..)

Now another seed I had gotten from my friend's stash of AfroBerry, which he had brought home from San Francisco over a summer vacation. This strain was (I believe, because I cant find much info) a Cali HydroMedical product..

ANYWAYS.. Down the road, after a few more dirt crops, I was gettin some good buds of these two different plants; AfroBerry and what I ended up calling Amber Kush. As I continued I noticed the quality increase and the genetics stabilize. However, I never had a mother plant, so the clones came from clones, which came from clones, etc, etc.. So the last clones from the dirt crop were getting converted to hydro, and thats when it all went to shit..

The AfroBerry after the first run through produced little to nothing of some fluffy little air nuggets, and the Amber Kush did a little better, but not by much. Down the line much further and the AfroBerry just seemed to get worse and worse. The nugget formation definitely got better, but before it was removed it was a very fickle plant, which easily went deficient, hermed out, and was very hard to clone. The Amber Kush, however, did redeem itself and become a worthy plant.

Turns out if you search for AfroBerry online, you dont get much info. I assume it got discontinued in the community because of its instability..

Sorry for the intense ramble but overall..

I guess it all depends on the initial genetics you start with..
If youve got a hardy, stable strain, I suppose with time it will get better (given the conditions remain the same/similar)..

But I also think if your original genetics are kinda tricky and complicated, it may have a harder time trying to adapt, all the while losing stability.

I could be totally wrong, but I think I saw that info in a magazine..
It wasnt about cannabis, but I am pretty sure the article mentioned something about trait loss or something similar.

I'll start lookin through my bookshelves and try to find it, so I can give you the reference

[Butcher Bob]

 

Originally Posted by TheHerbalEssence

...selectively breeding/cloning...

I think wut you are referrin to is genetic drift, and that is a term that is applied to breeding, not cloning. It has to do with losin stuff as the seeds are conceived. But the DNA blueprint does not change from clone to clone...period. Wut caused the changes you experienced, are changes to environment. The DNA blueprint allows fer different courses of action, based on the environment it encounters, but the programming itself does not change.

In breeding, it becomes important because the DNA learns & records environmental conditions...it does not use this information itself, but it will be passed on to future generations and affect how their DNA is constructed. For example, and I've done this many times, if you got a clone that is healthy and performs well, and then you put a succession of those clones thru hell (mites, drought, bad nutes, etc.), you kin still take a clone from a poorly kept plant, bring it back into it's initial environment, and it will return to it's original state. It works different in breeding. Let's say you take two clones from the same mother plant, one stays healthy and the other is exposed to powdery mildew. Those two plants will now produce differences in offspring, because the experience with the powdery mildew has bin recorded in the DNA of one, but not the other.

[TheHerbalEssence]

 

Wow Bob. Thanks for clearing that up. Im really glad you told me this. Some great info right there..

So essentially if I took a clone of my AfroBerry, and returned it to its original conditions in dirt, it would revert to how it grew back then?

Pretty sweeeeeet...

[Butcher Bob]

 

It should, yes. There is always the possibility of pathogens bein present now, that were not present then, and therefore giving you a "different" plant, even in the same environment. For example, powdery mildew is systemic, it invades and persists within the plant itself...so it would still be present, unless it's bin addressed and eliminated.

[TheHerbalEssence]

 

Ah, gotchya.

But I never had to deal with powdery mildew before.

Lets hope I never have to!

[YoungHead]

 

Originally Posted by Butcher Bob

Please explain this.....cuz I would disagree.

I was actually just reading about this
Not only can it happen with cuttings, but a mature plant can actually mutate and throw out a slightly genetically different branch. They are called "sports"
If you cloned that sport as your new mother...you have changed your clone

Its rare, but not that rare.

As far as genetics go, the rules are really more like guidelines. it usually will happen in predictable ways, but not always. After all if it didn't, asexual organisms wouldn't have evolved into us

[dequilo]

 

Its rare, but not that rare.

like when a variegated plants put out a non variegated branch or the other way around

it does happen more then you might think

but that is a tip cutting not a culture from a cell mass

all the best

dequilo

[YoungHead]

 

yeah a few of the well known commercial cuttings (non MJ) started out this way

the ruby red grapefruit is an example

[dequilo]

 

yeah a few of the well known commercial cuttings (non MJ) started out this way

an old drunk down in deerfield beach FLA found a sport on an Arboricola that made him a ton of dough

I forget the name of it

all the best and be safe

Dequilo

[Butcher Bob]

 

Originally Posted by YoungHead

..."sports"...

Typically found in woody plants. Way less common in annuals.